GM-CSF Protein
Quick Overview for GM-CSF Protein (ABIN987967)
Target
See all GM-CSF (CSF2) ProteinsBiological Activity
Origin
Source
Purity
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Characteristics
- Resuspend cells in assay media free of h-GM-CSF containing 10 % FBS and 2X Gentamicin. Transfer 100ml culture/well to assay plate (19,500 cells/well, passage # 13). Serial dilute h-GM-CSF in assay media in duplicate. Transfer 100 ml/well serial diluted h-GM-CSF to cells in assay plate. Final assay volume of each well is 200 ml containing 2X Gentamicin and h-GM-CSF. Incubate 70 hours. Add 20 ml of Promega Substrate Cell Titer 96 Aqueous One Solution Reagent to each well. Incubate 37°C and read OD at 490 nm. The ED50 for this effect is 0.06 - 0.09 ng/ml.
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Endotoxin Level
- level is less than 0.03 ng per myg (0.3 EU/myg) of human GMCSF
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Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-141) (Active) protein
CSF2 Origin: Mouse Host: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (Active) protein (His tag)CSF2 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (Active) proteinCSF2 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) protein (Fc Tag)CSF2 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) protein (His tag)CSF2 Origin: Rat Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 1-144) protein (GST tag)CSF2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-141) (Active) protein (His tag)CSF2 Origin: Mouse Host: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, ELISA, Imm, AcA Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) (Active) protein (His tag)CSF2 Origin: Human Host: HEK-293 Cells > 95 % AcA, CC Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- -20 °C
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- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
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Alternative Name
- Granulocyte Macrophage Colony Stimulating Factor
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Background
- GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. Synonym: rHuGM-CSF 7, hGM-CS. Formulation: Lyophilized from a 0.2 mym filtered solution in 10 mM PB, pH7.0.
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Pathways
- JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin
Target
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