IL13 Variant (Active) Protein
Origin: Human
Host: Escherichia coli (E. coli)
> 95 % by SDS-PAGE and HPLC analyses.
Active
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- Target
- IL13 Variant
- Biological Activity
- Active
- Origin
- Human
- Source
- Escherichia coli (E. coli)
- Sequence
- SPGPVPPSTA LRELIEELVN ITQNQKAPLC NGSMVWSINL TAGMYCAALE SLINVSGCSA IEKTQRMLSG FCPHKVSAGQ FSSLHVRDTK IEVAQFVKDL LLHLKKLFRE GQF
- Characteristics
- Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent proliferation of TF-1 cells was < 1.0 ng/ml, corresponding to a specific activity of > 1×106 units/mg. This analog has also been shown to exhibit increased in vivo activity compared to wild type IL-13.
- Purity
- > 95 % by SDS-PAGE and HPLC analyses.
- Endotoxin Level
- Level Less than 1EU/µg of rHuIL-13 variant as determined by LAL method
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.
- Storage
- 4 °C
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- Target
- IL13 Variant
- Alternative Name
- Interleukin-13 Variant (IL-13 V)
- Background
- The human IL-13 cDNA encodes a 132 amino acid protein containing a proposed 20 amino acid signal peptide. Human IL-13 shares approximately 30% amino acid sequence homology to human IL-4 and the two cytokines exhibit overlapping biological activities. Human IL-13 is produced by activated Th0, Th1-like Th2-like and CD8 T cells. Similarly to IL-4, IL-13 has multiple effects on the differentiation and functions of monocytes/macrophages. IL-13 can suppress the cytotoxic functions of monocytes/macrophages. It can also suppress the production of proinflammatory cytokines and upregulate the production of IL-1ra by monocytes/macrophages. Synonym: Interleukin-13 Variant ( IL-13 V), Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.2, containing 5% trehalose.
- Molecular Weight
- Approximately 12.5 kDa, a single non-glycosylated polypeptide chain containing 114 amino acids, with a substitution of Q for R at position 112 compared with the wild type IL-13.
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