IL-21 Protein

Catalog No. ABIN988060

Product Details

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Sequence: QGQDRHMIRM RQLIDIVDQL KNYVNDLVPE FLPAPEDVET NCEWSAFSCF QKAQLKSANT GNNERIINVS IKKLKRKPPS TNAGRRQKHR LTCPSCDSYE KKPPKEFLER FKSLLQKMIH QHLSSRTHGS ED

Characteristics: Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using human peripheral blood mononuclear cells is less than 10 ng/ml, corresponding to a specific activity of >, 1.0 × 105 IU/mg.

Purity: > 97 % by SDS-PAGE and HPLC analyses.

Endotoxin Level: Level Less than 1EU/µg of rHuIL-21 as determined by LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage: 4 °C

Target Details

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Alternative Name: Interleukin-21 (IL-21) (IL21 Products)

Background: IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. The biological effects of IL-21 include induction of differentiation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjuction) with IL-4 of IgG production, and induction of apoptotic effects in naïve B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the gamma chain (gamma,c) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. Synonym: Interleukin-21 (IL-21), Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

Molecular Weight: Approximately 15.4 kDa, a single non-glycosylated polypeptide chain containing 133 amino acids.

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response