PreScission Protease (PSP) protein (GST tag)
Origin: Enterovirus (EV71), Schistosoma japonicum
Host: Escherichia coli (E. coli)
Recombinant
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- Target
- PreScission Protease (PSP)
- Protein Type
- Recombinant
- Origin
- Enterovirus (EV71), Schistosoma japonicum
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- GST tag
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- Application Notes
- During cleavage reactions, it is recommended that samples be removed at various time points and analyzed by SDS-PAGE to estimate the yield, purity, and extent of digestion. The amount of PreScission Protease, temperature and length of incubation required for complete digestion of a given GST fusion partner may vary depending on the fusion partner. Optimal conditions for each fusion should be determined in pilot experiments. Digestion may be improved by adding TritonTM X-100, TweenTM 20, NonidetTM, or NP40 to a concentration of 0.01%. Concentrations of these detergents up to 1% do not inhibit PreScission Protease.
- Restrictions
- For Research Use only
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- Buffer
- Cleavage Buffer: 50mM Tris-HCl, pH7.0 (at 25°C), 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol. Chill to 5°C prior to use.
- Storage
- -20 °C
- Storage Comment
- Should be stored in small aliquots at -20°C to -80°C for long term.
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- Target
- PreScission Protease (PSP)
- Target Type
- Viral Protein
- Background
- PreScission Protease is a fusion protein of glutathione S-transferase (GST) and human rhinovirus (HRV) type 14 3C protease. The protease specifically recognizes a subset of sequences which include the core AA sequence Leu-Phe-Gln/Gly-Pro cleaving between the Gln and Gly residues. Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as well. Synonym: PreScission Protease(PSP). .
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