CD40 Ligand Protein (CD40LG) (Soluble)

Catalog No. ABIN988235

CD40 Ligand protein expressed in Escherichia coli (E. coli).

Quick Overview for CD40 Ligand Protein (CD40LG) (Soluble) (ABIN988235)

Target: CD40 Ligand (CD40LG)

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % by SDS-PAGE and HPLC analyses.

Product Details

Protein Characteristics: Soluble

Sequence: MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLK

Characteristics: Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using B cell-enriched peripheral blood mononuclear cells (PBMC) is less than 3000 ng/ml, corresponding to a specific activity of >, 3.3 × 102 IU/mg in the presence of 20ng/ml rHuIL4.

Endotoxin Level: Level Less than 1EU/µg of rHusCD40L as determined by LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage: 4 °C

Target Details

Target: CD40 Ligand (CD40LG)

Alternative Name: CD40 Ligand (sCD40L)

Background: CD40 ligand, CD40L (also known as CD154, TRAP or gp39), is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF family, CD40L is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, like NK cells, mast cells, basophils and eosinophils. Human CD40L shares 78% amino acid identity with its mouse counterpart. The receptor of CD40L is CD40, a type I transmembrane glycoprotein belonging to the TNF receptor family. CD40 is expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelium. Although all monomeric, dimeric and trimeric forms of soluble CD40L can bind to CD40, the trimeric form of soluble CD40L has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members. CD40L mediates a range of activities on B cells including induction of activation-associated surface antigen, entry into cell cycle, isotype switching and Ig secretion and memory generation. CD40-CD40L interaction also plays important roles in monocyte activation and dendritic cell maturation. Synonym: soluble CD40 Ligand ( sCD40L), Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.0.

Molecular Weight: Approximately 16.3 kDa. a single non-glycosylated polypeptide chain containing 149 amino acids.

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints