Selenoprotein F Protein (SELENOF) (AA 29-165) (His tag)
This Recombinant Selenoprotein F protein is expressed in Escherichia coli (E. coli).
Catalog No. ABIN7676652
Quick Overview for Selenoprotein F Protein (SELENOF) (AA 29-165) (His tag) (ABIN7676652)
Target
Selenoprotein F (SELENOF)
Protein Type
Recombinant
Origin
Human
Source
Escherichia coli (E. coli)
Application
SDS-PAGE (SDS)
Purity
> 95 %
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Protein Characteristics
- AA 29-165
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Purification tag / Conjugate
- This Selenoprotein F protein is labelled with His tag.
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Sequence
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VSAFGAE FSSEACRELG FSSNLLCSSC DLLGQFNLLQ LDPDCRGCCQ EEAQFETKKL YAGAILEVCG CKLGRFPQVQ AFVRSDKPKL FRGLQIKYVR GSDPVLKLLD DNGNIAEELS ILKWNTDSVE EFLSEKLERI -
Purification
- > 95% by SDS-PAGE
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- Selenoprotein F (SELENOF)
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Alternative Name
- Seleno F
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Background
- SEP15 is a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the uGA codon that normally signals translation termination. The 3' uTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of uGA as a Sec codon rather than as a stop signal. Studies in mouse suggest that this selenoprotein may have redox function and may be involved in the quality control of protein folding. This gene is localized on chromosome 1p31, a genetic locus commonly mutated or deleted in human cancers. Recombinant human SEP15 (SC96C) protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 17.7 kDa (160aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_004252
Target
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