Goat anti-Cow IgG (Heavy & Light Chain) Antibody

Catalog No. ABIN100885

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Cow

Host: Goat

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: IgG (H&L)

Cross-Reactivity: Cow (Bovine)

Characteristics: Anti-Bovine IgG Secondary Antibodies are designed for Western Blotting, ELISA and Immunohistochemistry. Secondary antibodies can also be used for a variety of other applications such as Assay Development, Flow Cytometry and in situ hybridization. C
oncentration Definition: by Refractometry

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, Bovine IgG and Bovine Serum.

Immunogen:

Immunogen: Bovine IgG whole molecule

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Bovine IgG Secondary Antibodies are designed for Western Blotting, ELISA and Immunohistochemistry. Secondary antibodies can also be used for a variety of other applications such as Assay Development, Flow Cytometry and in situ hybridization.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 28.5 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Preservative: Without preservative

Storage: RT,4 °C,-20 °C

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Goat Anti-Bovine IgG Antibody, Secondary Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.