Donkey anti-Goat IgG (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed

Catalog No. ABIN101180

Supplier Product No.: 605-706-125

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Goat

Host: Donkey

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Supplier Product No.: 605-706-125

Supplier: Rockland

Purpose: Goat IgG (H&L) Antibody Biotin Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Ch GP Ham Hs Ms Rb & Rt Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Donkey Serum, Goat IgG and Goat Serum.  No reaction was observed against Chicken, Guinea Pig, Horse, Hamster, Mouse, Rabbit and Rat Serum Proteins.

Characteristics: Anti-Goat IgG Biotin Antibody generated in donkey detects goat IgG.

Purification: Anti-Goat IgG (H&L) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Anti-Goat IgG (H&L) was produced by repeated immunization with goat IgG whole molecule in donkey.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Application Note: Goat IgG Biotin Conjugated Antibody has been tested by ELISA and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:60,000 ELISA Dilution: 1:600,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References

Yamashita, Ohmoto, Yamaguchi, Matsumoto, Hirota: "Skn-1a/Pou2f3 functions as a master regulator to generate Trpm5-expressing chemosensory cells in mice." in: PLoS ONE, Vol. 12, Issue 12, pp. e0189340, (2018) (PubMed).

He, Qiu, Dai, Fang, Sun, Gong, Ding, Sun: "MicroRNA-351 inhibits denervation-induced muscle atrophy by targeting TRAF6." in: Experimental and therapeutic medicine, Vol. 12, Issue 6, pp. 4029-4034, (2016) (PubMed).

Tsoulis, Chang, Moore, Chan, Gohir, Petrik, Vickers, Connor, Sloboda: "Maternal High-Fat Diet-Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring." in: Biology of reproduction, Vol. 94, Issue 4, pp. 94, (2016) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Anti-Goat IgG (H&L) Antibody is ideal for researchers in Immunology, Cancer, and Microbiology research.