Rabbit anti-Goat IgG (Fc Region) Antibody (HRP)

Catalog No. ABIN101255

Supplier Product No.: 605-4303

Quick Overview for Rabbit anti-Goat IgG (Fc Region) Antibody (HRP) (ABIN101255)

Target: IgG

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: Fc Region

Reactivity: Goat

Host: Rabbit

Supplier Product No.: 605-4303

Supplier: Rockland

Purpose: Goat IgG Fc Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG, Goat IgG F(c) and Goat Serum. No reaction was observed against Goat IgG F(ab) .

Characteristics: Anti-Goat IgG Peroxidase Antibody generated in rabbit detects goat IgG.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads.

Immunogen:

Immunogen: Anti-Goat IgG F(c) was produced by repeated immunization with Goat IgG F(c) fragment in rabbit.

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Goat IgG F(c) peroxidase conjugated antibody has been tested by ELISA and is assayed against 1.0 μg of Goat IgG in a standard capture ELISA using TMB as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:16,000 is suggested for this product. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:10,000 - 1:30,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Ma, Yan, Huang, Zhao, Zhu: "Downregulation of human epidermal growth factor receptor 2 by short hairpin RNA increases chemosensitivity of human ovarian cancer cells." in: Oncology letters, Vol. 9, Issue 5, pp. 2211-2217, (2015) (PubMed).

Target Details

Target: IgG

Target Type: Antibody

Background: Anti-Goat IgG F(ab')2 Peroxidase Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind goat IgG F(c) will not bind goat IgG F(ab')2 Molecules.