Rabbit anti-Human IgG (Heavy & Light Chain) Antibody - Preadsorbed
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- Target See all IgG products
- IgG
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Specificity
- IgG (H&L)
- Cross-Reactivity
- Human
- Characteristics
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Anti-Human antibody generated in rabbit detects specifically human IgG (H&L). This secondary antibody anti-Human is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm - Purification
- Preadsorption: Solid phase absorption
- Sterility
- Sterile filtered
- Immunogen
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Immunogen: Anti-Human IgG was produced by repeated immunization with human IgG whole molecule fragment in rabbit.
Immunogen Type: Native Protein
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry Dilution: 1:1,000 - 1:10,000
Application Note: Anti-Human IgG (H&L) is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
ELISA Dilution: 1:75,000 - 1:500,000
Western Blot Dilution: 1:10,000 - 1:50,000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 2.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Expiry Date
- 12 months
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
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Synonyms: Human IgG (H&L) Antibody, Rabbit-a-Human IgG (H&L), Human IgG (H&L) Antibody in rabbit, Rabbit anti-human IgG Secondary Antibody.
Background: Anti-Human IgG (H&L) generated in rabbit detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present. It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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