Rabbit anti-Pig IgG (F(ab')2 Region) Antibody (FITC)

Catalog No. ABIN101898

Supplier Product No.: 214-4204

Quick Overview for Rabbit anti-Pig IgG (F(ab')2 Region) Antibody (FITC) (ABIN101898)

Target: IgG

Clonality: Polyclonal

Conjugate: FITC

Application: Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Isotype: IgG

Product Details

Binding Specificity: F(ab')2 Region

Reactivity: Pig

Host: Rabbit

Supplier Product No.: 214-4204

Supplier: Rockland

Purpose: Swine IgG F(ab')2 Antibody Fluorescein Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Swine IgG, Swine IgG F(ab')2 and Swine Serum.  No reaction was observed against Swine IgG F(c).

Characteristics: Anti-Swine IgG (H&L) Fluorescein Antibody generated in goat detects reactivity to swine IgG.

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen: Optional[Immunogen]: Swine IgG F(ab')2 fragment

Application Details

Application Notes: Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody

Background: Anti-Swine IgG F(ab')2 Fluorescein Antibody generated in rabbit recognizes the dimeric Fab portion of the swine IgG molecule. Swine IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind swine IgG F(c) will not bind swine IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Swine IgG F(ab')2 antibody is conjugated to Fluorescein.