Rabbit anti-Sheep IgG (Fc Region) Antibody - Preadsorbed

Catalog No. ABIN102297

Product Details

Target: IgG

Binding Specificity: Fc Region

Reactivity: Sheep

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: IgG F(c)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: immunoaffinity chromatography using Sheep IgG coupled to agarose beads

Sterility: Sterile filtered

Immunogen:

Immunogen: Sheep IgG F(c) fragment

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Sheep IgG F(c) antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Rabbit Anti-Sheep IgG F(c) Antibody, Rabbit Anti-Sheep IgG Fc Antibody, Rabbit Anti-Sheep IgG Fc Fragment Antibody

Background: Anti-Sheep IgG F(c) generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of sheep IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.