Mouse anti-Goat IgG (Heavy & Light Chain) Antibody (TRITC)

Catalog No. ABIN1043935

Supplier Product No.: 605-300-002

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Goat

Host: Mouse

Clonality: Polyclonal

Conjugate: TRITC

Application: Fluorescence Microscopy (FM), Dot Blot (DB)

Supplier Product No.: 605-300-002

Supplier: Rockland

Purpose: Goat IgG (H&L) Antibody Rhodamine Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Goat IgG and Goat Serum.  No reaction was observed against Rabbit, Human and Mouse Serum Proteins.

Purification: Goat IgG (H&L) Antibody Rhodamine Conjugated was prepared from monospecific polyclonal ascites by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Optional[Immunogen]: Goat IgG whole molecule

Isotype: IgG

Application Details

Application Notes: Application Note: Mouse Anti-Goat IgG (H&L) Rhodamine Conjugated Antibody has been tested by dot blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. IF Microscopy Dilution: 1:500-1:2,500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.