Goat anti-Mouse IgG2a Antibody

Catalog No. ABIN1043978

Supplier Product No.: 610-101-041

Quick Overview for Goat anti-Mouse IgG2a Antibody (ABIN1043978)

Target: IgG2a

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Goat

Supplier Product No.: 610-101-041

Supplier: Rockland

Purpose: Mouse IgG2a Antibody Pre-adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against to Bv, Hu & Rb Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum, Mouse IgG and Mouse IgG2a. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA for human and rabbit. For IgG1, IgG2b and IgG3 reactivity was less than 5 % against target (IgG2a, IgG). Cross-reactivity against Bovine is assessed only by IEP.

Characteristics: Anti-Mouse IgG1 Antibody generated in goat detects reactivity to Mouse IgG1 (Gamma 1 chain).

Purification: MOUSE IgG2a Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG2a coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen:

Immunogen: Mouse IgG2a heavy chain

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Mouse IgG2a secondary antibody has been tested by ELISA and dot blot and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG2a antibody based assays. Mouse IgG2a secondary antibody is available in a variety of formats. Immunohistochemistry Dilution: 1:2,000 - 1:10,000 Western Blot Dilution: 1:4,000 - 1:20,000 ELISA Dilution: 1:40,000 - 1:200,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.1 mg/mL

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details

Target: IgG2a

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.