Goat anti-Rat IgG2b (Subclass specific) Antibody (TRITC)

Catalog No. ABIN1099730

Quick Overview for Goat anti-Rat IgG2b (Subclass specific) Antibody (TRITC) (ABIN1099730)

Target: IgG2b

Clonality: Polyclonal

Conjugate: TRITC

Application: ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Indirect Immunofluorescence Assay (IFA)

Product Details

Binding Specificity: Subclass specific

Reactivity: Rat

Host: Goat

Purpose: Goat anti Rat IgG2b (subclass specific), conjugated with TRITC

Specificity: Tetramethylrhodamine isothiocyanate-conjugated IgG fraction of polyclonal Goat antiserum to rat IgG2b, subclass specific.

Cross-Reactivity (Details): This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this antiSerum has not been tested in detail, however in double radial immunodiffusion a weak reaction with Mouse has been observed.

Characteristics: The reactivity of the antiserum is directed to the subclass IgG2b. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining for the detection of IgG2b at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to identify and measure IgG2b in rat serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Purification: Purified IgG fraction from antiserum

Immunogen: Goats were immunized with pools of purified homogenous IgG2b isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: TRITC-coupled It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: TRITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Target Details

Target: IgG2b

Target Type: Antibody