Goat anti-Chicken IgY (Heavy & Light Chain) Antibody (DyLight 633)

Catalog No. ABIN1110362

Quick Overview for Goat anti-Chicken IgY (Heavy & Light Chain) Antibody (DyLight 633) (ABIN1110362)

Target: IgY

Clonality: Polyclonal

Conjugate: DyLight 633

Application: Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Chicken

Host: Goat

Predicted Reactivity: chicken IgY heavy and light chains (H&L)

Characteristics: Goat anti-chicken IgY, DyLight® 633 Conjugate is a secondary antibody conjugated to DyLight® 633, which binds to hen immunoglobulins in immunological assays.

Purification: Affinity purification

Immunogen: purified chicken IgY, whole molecule

Application Details

Application Notes: 1:20 - 1:2 000 (IHC), 1:50 - 1:5 000 (ICC)

Comment:

Based on immunoelectrophoresis this antibody reacts with heavy chains on chicken IgG and light chains on all chicken immunoglobulins.No reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.

Handling Advice: Product is photosensitive and should be protected from light.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Target Details

Target: IgY

Target Type: Antibody