Goat anti-Mouse IgG1 Antibody (Alkaline Phosphatase (AP)) - Preadsorbed

Catalog No. ABIN1118472

Supplier Product No.: 610-105-040

Quick Overview for Goat anti-Mouse IgG1 Antibody (Alkaline Phosphatase (AP)) - Preadsorbed (ABIN1118472)

Target: IgG1

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Goat

Supplier Product No.: 610-105-040

Supplier: Rockland

Purpose: Mouse IgG1 Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Bv, Hu, and Rb Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Mouse Serum, Mouse IgG and Mouse IgG1. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1 % of target signal.

Characteristics: Anti-Mouse IgG IgA IgM Peroxidase Antibody generated in goat detects reactivity to Mouse IgG, Mouse IgA, and Mouse IgM.

Purification: Anti-Mouse IgG1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG1 coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen:

Immunogen: Mouse IgG1 heavy chain

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Mouse IgG1 Alkaline Phosphatase Conjugated Secondary antibody has been tested by ELISA and dot blot and is ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:10,000 - 1:40,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C before opening. DO NOT FREEZE. Conjugated antibody is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Expiry Date: 12 months

Target Details

Target: IgG1

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.