Goat anti-Mouse IgG3 Antibody (Biotin)

Catalog No. ABIN1118507

Supplier Product No.: 610-106-043

Quick Overview for Goat anti-Mouse IgG3 Antibody (Biotin) (ABIN1118507)

Target: IgG3

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Goat

Supplier Product No.: 610-106-043

Supplier: Rockland

Purpose: Mouse IgG3 Antibody Biotin Conjugated Pre-adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Bv, Hu, and Rb Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Mouse Serum and Mouse IgG3. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1 % of target signal.

Characteristics: Anti-Mouse IgG2b Biotin Antibody generated in goat detects reactivity to Mouse IgG2b (Gamma 2b chain).

Purification: Anti-MOUSE IgG3 (Gamma 3 chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Mouse IgG3 heavy chain

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Mouse IgG3 secondary antibody is available in a variety of formats. Anti-Mouse IgG3 secondary antibody is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG3 antibody based assays. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store second antibody at 4° C prior to restoration. For extended storage aliquot secondary antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: IgG3

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG3 comprises almost 10 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.