Rabbit anti-Horse IgG (Whole Molecule) Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN117211

Quick Overview for Rabbit anti-Horse IgG (Whole Molecule) Antibody (Alkaline Phosphatase (AP)) (ABIN117211)

Target: IgG

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Product Details

Binding Specificity: Whole Molecule

Reactivity: Horse

Host: Rabbit

Purification: Immunoaffinity chromatography.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads.

Immunogen: Horse IgG whole molecule.

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry aswell as other phosphatase-antibody based enzymatic assays requiring lot-to-lotconsistency. This product has been assayed against 1.0 μg of Horse IgG in a standardcapture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at roomtemperature. A working dilution of 1:6,000 to 1:30,000 of the reconstitution concentrationis suggested for this product.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: 0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.005 M Magnesium Chloride, 0.0005 M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0, with 10 mg/mL BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Target Details

Target: IgG

Target Type: Antibody

Background: Synonyms: Horse IgG, Horse Immunoglobulin G