Rabbit anti-Human IgG3 Antibody (FITC)

Catalog No. ABIN1173722

Quick Overview for Rabbit anti-Human IgG3 Antibody (FITC) (ABIN1173722)

Target: IgG3

Clonality: Polyclonal

Conjugate: FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Isotype: IgG

Product Details

Reactivity: Human

Host: Rabbit

Specificity: It has been selected for its ability to recognize IgG3 in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: The antibody is a rabbit polyclonal antibody raised against IgG3 conjugated to fitc.

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Comment:

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 200 µg/mL

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details

Target: IgG3

Target Type: Antibody