Goat anti-Human IgG (Whole Molecule) Antibody (PE) - Preadsorbed

Catalog No. ABIN117679

Product Details

Target: IgG

Binding Specificity: Whole Molecule

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: PE

Application: Flow Cytometry (FACS), Immunomicroscopy (IM)

Characteristics: Molar Ratio: 490 nm, 545 nm and 565 nm / 580 nm

Purification: Preadsorption: Solid phase absorption

Immunogen: Human IgG whole molecule

Application Details

Application Notes: Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximumamount of reagent required to stain 1 x 10^6 cells in flow cytometry is approximately 1.0 gof antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Recommended dilutions: Flow cytometry: 1:100 - 1:200IF Mocroscopy: 1:100 - 1:200

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Comment:

Excitation/Emission wavelength: 496 nm/576 nm

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent).

Concentration: 0.5 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.05 % (w/v) Sodium Azide as preservative and 10 mg/mL Bovine Serum Albumin (BSA), IgG and Protease free, as stabilizer.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -24 °C or below. This antibody is stable for one month at 4 °C as an undiluted liquid.

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Synonyms: Human Immunoglobulin G