Goat anti-Human IgM (Fc5mu Region) Antibody (FITC) - Preadsorbed

Catalog No. ABIN117707

Quick Overview for Goat anti-Human IgM (Fc5mu Region) Antibody (FITC) - Preadsorbed (ABIN117707)

Target: IgM

Clonality: Polyclonal

Conjugate: FITC

Application: Flow Cytometry (FACS), Immunomicroscopy (IM)

Product Details

Binding Specificity: Fc5mu Region

Reactivity: Human

Host: Goat

Characteristics: Molar Ratio: 2.7 moles FITC per mole of Goat IgG F(ab')2.

Purification: Preadsorption: Solid phase absorption

Immunogen: Human IgM Fc5µ fragment.

Application Details

Application Notes: Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Flow Cytometry: 1:2,000-1:10,000. Immunofluorescence microscopy: 1:500-1:2,500.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Comment:

Absorption / Emission: 495 nm / 528 nm

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.5 mL of deionized water (or equivalent)

Concentration: 0.75 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Product is photosensitive and should be protected from light.
Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C before opening. This product is stable at 4 °C as an undiluted liquid.

Target Details

Target: IgM

Target Type: Antibody

Background: Synonyms: Human Immunoglobulin M