Mouse anti-Rat IgE (Chain epsilon) Antibody (Biotin)
WB, EIA
Monoclonal
MARE-1
IgG1
Biotin
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- Target See all IgE products
- IgE
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Binding Specificity
- Chain epsilon
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Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Biotin
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Purification
- Affinity chromatography on IR2 rat IgE myeloma
- Immunogen
- IR162, IR1016, IR2 and IR410 rat IgE myeloma proteins. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Clone
- MARE-1
- Isotype
- IgG1
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- Application Notes
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ELISA: 5 μg/mL as detecting antibody. Western Blot.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS containing 0.09 % Sodium Azide and 50 % Glycerol
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
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- Target
- IgE
- Abstract
- IgE Products
- Background
- Synonyms: Rat Immunoglobulin E
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