Mouse anti-Chicken IgY (Heavy Chain) Antibody
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- Target See all IgY products
- IgY
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Binding Specificity
- Heavy Chain
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Reactivity
- Chicken
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Host
- Mouse
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Clonality
- Monoclonal
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
- Purification
- Size exclusion chromatography
- Immunogen
- Chicken egg yolk immunoglobulin (IgY) Epitope IgY heavy chain
- Clone
- 4G12
- Isotype
- IgG1
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- Application Notes
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Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL. Immunoprecipitation: 1 - 10 g.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore with 1 mL H2O (15 min, RT)
- Buffer
- 1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C/-80 °C
- Storage Comment
- Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Thaw aliquots at 30 °C. Thawed aliquots may be stored at 0 °C up to 3 months.
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- Target
- IgY
- Alternative Name
- IgG + IgY (IgY Products)
- Background
- Synonyms: Chicken Immunoglobulin G, Chicken Immunoglobulin Y
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