Mouse anti-Human IgG (Fc Region) Antibody (HRP)

Catalog No. ABIN135620

Quick Overview for Mouse anti-Human IgG (Fc Region) Antibody (HRP) (ABIN135620)

Target: IgG

Clonality: Monoclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Clone: JDC-10

Isotype: IgG1

Product Details

Binding Specificity: Fc Region

Reactivity: Human

Host: Mouse

Specificity: Reacts with the Fc portion of the heavy chain of all subclasses of human IgG as demonstrated by ELISA.

Purification: Immunoaffinity chromatography

Application Details

Application Notes: Working Dilution:
ELISA: 1:1,000-1:2,000
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 1.0 mL of stock solution in 50 % glycerol / 50 % PBS, pH 7.4.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C

References

Roman, Qiu, Dornadula, Hamuro, Bakhtiar, Verch: "Application of miniaturized immunoassays to discovery pharmacokinetic bioanalysis." in: Journal of pharmacological and toxicological methods, (2010) (PubMed).

Target Details

Target: IgG

Target Type: Antibody