Mouse anti-Human IgG1 (Hinge Region) Antibody (HRP)

Catalog No. ABIN135625

Quick Overview for Mouse anti-Human IgG1 (Hinge Region) Antibody (HRP) (ABIN135625)

Target: IgG1

Clonality: Monoclonal

Conjugate: HRP

Application: ELISA

Clone: 4E3

Isotype: IgG1

Product Details

Binding Specificity: Hinge Region

Reactivity: Human

Host: Mouse

Purification: Protein A affinity column

Application Details

Application Notes: Working Dilution:
ELISA: 1:2,000-1:8,000
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 1.0 mL of stock solution in 50 % glycerol / 50 % PBS, pH 7.4.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C

References

Haag, Schneider, Mason, Tuncel, Andersson, Peters, Burkhardt, Holmdahl: "Mass spectrometric analysis of citrullinated type II collagen reveals new citrulline-specific autoantibodies, which bind to human arthritic cartilage." in: Arthritis & rheumatology (Hoboken, N.J.), (2014) (PubMed).

Target Details

Target: IgG1

Target Type: Antibody