Mouse anti-Human IgG4 (pFc' Region) Antibody

Catalog No. ABIN135656

Quick Overview for Mouse anti-Human IgG4 (pFc' Region) Antibody (ABIN135656)

Target: IgG4

Clonality: Monoclonal

Application: ELISA

Clone: HP6023

Isotype: IgG3

Product Details

Binding Specificity: pFc' Region

Reactivity: Human

Host: Mouse

Specificity: Reacts with the pFc' portion of the heavy chain of human IgG4 as demonstrated by ELISA

Characteristics: Mouse Anti-Human IgG4 pFc'-UNLB

Purification: Purified

Immunogen: Human IgG4 myeloma protein

Application Details

Application Notes:

• Applications: ELISA - Quality tested , FLISA - Quality tested FC - Reported in literature , IHC-FS - Reported in literature , WB - Reported in literature , IP - Reported in literature , SPR - Reported in literature
• Working Dilutions: ELISA AP conjugate 1:1,000 - 1:2,000 HRP conjugate 1:2,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA FITC and AF488 conjugates 1:200 - 1:400 AF647 conjugate ≤ 2 g/mL PE conjugate ≤ 1 g/mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Dogan, Kozhaya, Placek, Gunter, Yigit, Hardy, Plassmeyer, Coatney, Lillard, Bukhari, Kleinberg, Hayes, Arditi, Klapper, Merin, Liang, Gupta, Alpan, Unutmaz: "SARS-CoV-2 specific antibody and neutralization assays reveal the wide range of the humoral immune response to virus." in: Communications biology, Vol. 4, Issue 1, pp. 129, (2021) (PubMed).

Haag, Schneider, Mason, Tuncel, Andersson, Peters, Burkhardt, Holmdahl: "Mass spectrometric analysis of citrullinated type II collagen reveals new citrulline-specific autoantibodies, which bind to human arthritic cartilage." in: Arthritis & rheumatology (Hoboken, N.J.), (2014) (PubMed).

Target Details

Target: IgG4

Target Type: Antibody