Sheep anti-Human IgG4 Antibody

Catalog No. ABIN458176

Product Details

Target: IgG4

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)

Characteristics: The activity of the antiserum is directed to human IgG4 subclass.
Precipitating polyclonal sheep antisera to human IgG4, subclass specific
Total protein and IgG concentration in the antiserum are comparable to those of pooled sheep serum. Antibody titre: The antigenic differences between subclasses are considerably less pronounced than those between classes or betw

Purification: Delipidated, heat inactivated lyophilized stable whole serum
Immunoaffinity adsorption with insolubilized highly purified myeloma proteins of the other subclass or other serum proteins as required.

Immunogen: Purified homogenous IgG4 is isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In precipitating techniques as immunoelectrophoresis and double radial immunodiffusion to identify and measure the concentration of IgG4 subclass in serum or other body fluids. Human IgG constitutes about 75 % of the total Immunoglobulins in the serum of normal adults. It consists of four subclasses which are distinguished in isotypic heterogeneity of the gamma chains, and in physicochemical, biological and effector properties. The term

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water.

Buffer: No foreign proteins added.

Preservative: Without preservative

Handling Advice: Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgG4

Alternative Name: Immunoglobulin G4 (IgG4) (IgG4 Products)

Target Type: Antibody