Mouse anti-Human IgG4 (Fc Region) Antibody

Catalog No. ABIN135660

Quick Overview for Mouse anti-Human IgG4 (Fc Region) Antibody (ABIN135660)

Target: IgG4

Clonality: Monoclonal

Application: ELISA

Clone: HP6025

Isotype: IgG1

Product Details

Binding Specificity: Fc Region

Reactivity: Human

Host: Mouse

Specificity: Reacts with the Fc portion of the heavy chain of human IgG4 as demonstrated by ELISA

Characteristics: Mouse Anti-Human IgG4 Fc-UNLB

Purification: Purified

Immunogen: Human IgG4 myeloma protein

Application Details

Application Notes:

• Applications: ELISA - Quality tested , FLISA - Quality tested FC - Reported in literature , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , WB - Reported in literature , Microarray - Reported in literature , Multiplex - Reported in literature , Purification - Reported in literature
• Working Dilutions: ELISA AP conjugate 1:1,000 - 1:2,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA FITC, AF488, and AF555 conjugates 1:200 - 1:400 PE and AF647 conjugates ≤ 1 g/mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Gao, Moore, Porcheray, Rong, Abidoglu, DeVito, Paine, Girouard, Saidman, Schoenfeld, Levin, Wong, Elias, Schuetz, Rosales, Fu, Zorn: "Pretransplant IgG Reactivity to Apoptotic Cells Correlates With Late kidney Allograft Loss." in: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, Vol. 14, Issue 7, pp. 1581-91, (2014) (PubMed).

Xu, Wang, Deng, Hu, Li, Chen, Ning, Wu, Huang, Li, Wu, Yu, Li: "The serological diagnosis of human clonorchiasis by time-resolved fluoroimmunoassay based on GST2-specific IgG4 detection." in: Parasitology research, (2013) (PubMed).

Harada, Shimoda, Kimura, Sato, Ikeda, Igarashi, Ren, Sato, Nakanuma: "Significance of IgG4-positive cells in extrahepatic cholangiocarcinoma: Molecular mechanism of IgG4 reaction in cancer tissue." in: Hepatology (Baltimore, Md.), (2012) (PubMed).

Jackson, Lucas, Melancon, Desai: "Clinical relevance and IgG subclass determination of non-HLA antibodies identified using endothelial cell precursors isolated from donor blood." in: Transplantation, Vol. 92, Issue 1, pp. 54-60, (2011) (PubMed).

Xu, Chen, Zhao: "High potential to reverse the inhibition of myeloperoxidase by ceruloplasmin of anti-myeloperoxidase autoantibodies of IgG3 subclass." in: Autoimmunity, (2011) (PubMed).

Xu, Chen, Cui, Zhao: "Influence of myeloperoxidase by anti-myeloperoxidase antibodies and its association with the disease activity in microscopic polyangiitis." in: Rheumatology (Oxford, England), Vol. 49, Issue 11, pp. 2068-75, (2010) (PubMed).

Target Details

Target: IgG4

Target Type: Antibody