Goat anti-Mouse IgG (Heavy & Light Chain) Antibody (Cy3)

Catalog No. ABIN2176527

Quick Overview for Goat anti-Mouse IgG (Heavy & Light Chain) Antibody (Cy3) (ABIN2176527)

Target: IgG

Clonality: Polyclonal

Conjugate: Cy3

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Goat

Purpose: Goat Anti-Mouse IgG Antibody (H+L), Cy3 Conjugated

Cross-Reactivity: Mouse

Purification: Purified by Protein A.

Application Details

Application Notes: WB(1:300-5000), FCM(1:20-100), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

References

Han, Wang, Duan: "Biofunctional polyelectrolytes assembling on biosensors - A versatile surface coating method for protein detections." in: Analytica chimica acta, Vol. 964, pp. 170-177, (2017) (PubMed).

Wu, Liu, Jiang, Chen, Wang, Zhu, Li: "Kindlin-2 siRNA inhibits vascular smooth muscle cell proliferation, migration and intimal hyperplasia via Wnt signaling." in: International journal of molecular medicine, Vol. 37, Issue 2, pp. 436-44, (2016) (PubMed).

Nie, Wang, Zhang, Cheng, Lv, Liu, Wang: "Multifunctional Assembly of Micrometer-Sized Colloids for Cell Sorting." in: Small (Weinheim an der Bergstrasse, Germany), (2015) (PubMed).

Lv, Li, Liu, Li, Liu, Shen, Wang, Xue, Cao: "Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus." in: Journal of veterinary science, Vol. 15, Issue 2, pp. 209-16, (2015) (PubMed).

Gao, Zhao, Song, Yang: "Expression pattern of embryonic stem cell markers in DFAT cells and ADSCs." in: Molecular biology reports, Vol. 39, Issue 5, pp. 5791-804, (2012) (PubMed).

Target Details

Target: IgG

Target Type: Antibody

Background: Synonyms: Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions, to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.