Mouse anti-Rabbit IgM (Heavy & Light Chain) Antibody (AbBy Fluor® 488)

Catalog No. ABIN2176904

Quick Overview for Mouse anti-Rabbit IgM (Heavy & Light Chain) Antibody (AbBy Fluor® 488) (ABIN2176904)

Target: IgM

Clonality: Polyclonal

Conjugate: AbBy Fluor® 488

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Isotype: IgM

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Mouse

Purpose: Mouse Anti-Rabbit IgM Antibody (H+L), AbBy Fluor® 488 Conjugated

Cross-Reactivity: Rabbit

Purification: Purified by Protein A.

Application Details

Application Notes: WB(1:300-5000), FCM(1:20-100), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

References

Li, Liu, Qu, Li, Gao, Zhang: "Twinfilin 1 enhances milk bio-synthesis and proliferation of bovine mammary epithelial cells via the mTOR signaling pathway." in: Biochemical and biophysical research communications, Vol. 492, Issue 3, pp. 289-294, (2017) (PubMed).

Target Details

Target: IgM

Target Type: Antibody

Background: Synonyms: Immunoglobulin IgM normally constitutes about 10 % of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells.