Mouse anti-Rat kappa Light Chain Antibody (Biotin)
FACS, ELISA, IHC (fro)
Monoclonal
MRK
IgG1
Biotin
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- Target See all kappa Light Chain products
- kappa Light Chain
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Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
-
Conjugate
- Biotin
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Application
- Flow Cytometry (FACS), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))
- Brand
- BD Pharmingen™
- Characteristics
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The MRK-1 antibody reacts specifically with rat Igs bearing κ light chain. It does not react with λ light chain or heavy chains. MRK-1 antibody is effective for detection of cell-surface Ig by immunofluorescent staining with flow cytometric analysis. It has not been shown to stimulate B-cell proliferation.
BD Pharmingen™ Biotin Mouse Anti-Rat Ig, κ Light Chain - Biotin - Clone MRK-1 - Isotype Mouse IgG1 - Reactivity Rat - 0.5 mg - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Pooled rat Ig
- Clone
- MRK
- Isotype
- IgG1
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- Application Notes
- Flow Cytometry : Biotin-conjugated MRK-1 mAb may be used as a primary or secondary reagent in immunofluorescent staining. Immunohistochemistry: For use as a second-step in IHC, we suggest RG7/7.6 mAb (anti-rat and -hamster Ig κ light chain) in our special formulation for immunohistochemistry, Cat. No. 550336.
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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- Target
- kappa Light Chain
- Alternative Name
- Ig, kappa Light Chain (kappa Light Chain Products)
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