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Mouse anti-Rat kappa Light Chain Antibody (Biotin)

FACS, ELISA, IHC (fro) Monoclonal MRK IgG1 Biotin
Catalog No. ABIN2689605
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Quick Overview for Mouse anti-Rat kappa Light Chain Antibody (Biotin) (ABIN2689605)

Target

kappa Light Chain

Clonality

  • 47
  • 46
Monoclonal

Conjugate

  • 15
  • 10
  • 10
  • 9
  • 8
  • 4
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Biotin

Application

  • 63
  • 48
  • 44
  • 39
  • 37
  • 14
  • 10
  • 9
  • 9
  • 8
  • 8
  • 7
  • 5
  • 3
  • 1
Flow Cytometry (FACS), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone

MRK

Isotype

IgG1
  • Reactivity

    • 74
    • 19
    Rat

    Host

    • 44
    • 24
    • 14
    • 4
    • 4
    • 3
    Mouse

    Characteristics

    The MRK-1 antibody reacts specifically with rat Igs bearing κ light chain. It does not react with λ light chain or heavy chains. MRK-1 antibody is effective for detection of cell-surface Ig by immunofluorescent staining with flow cytometric analysis. It has not been shown to stimulate B-cell proliferation.

    BD Pharmingen™ Biotin Mouse Anti-Rat Ig, κ Light Chain - Biotin - Clone MRK-1 - Isotype Mouse IgG1 - Reactivity Rat - 0.5 mg

    Purification

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

    Immunogen

    Pooled rat Ig
  • Application Notes

    Flow Cytometry : Biotin-conjugated MRK-1 mAb may be used as a primary or secondary reagent in immunofluorescent staining. Immunohistochemistry: For use as a second-step in IHC, we suggest RG7/7.6 mAb (anti-rat and -hamster Ig κ light chain) in our special formulation for immunohistochemistry, Cat. No. 550336.

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

    Storage

    4 °C

    Storage Comment

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
  • Target

    kappa Light Chain

    Alternative Name

    Ig, kappa Light Chain
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