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Chicken anti-Rat IgG (Whole Molecule) Antibody (Alkaline Phosphatase (AP))

Name: Chicken anti-Rat IgG (Whole Molecule) Antibody (Alkaline Phosphatase (AP))
SKU: ABIN301020
Availability: InStock

ELISA, IHC, WB Polyclonal Alkaline Phosphatase (AP)

Catalog No. ABIN301020

$618.20

Plus shipping costs $50.00

1000 μg

Shipping to: United States

Delivery in 11 to 15 Business Days

Quick Overview for Chicken anti-Rat IgG (Whole Molecule) Antibody (Alkaline Phosphatase (AP)) (ABIN301020)

Target

IgG

Clonality

Polyclonal

Conjugate

Alkaline Phosphatase (AP)

Application

2620
2555
2306
1838
1470
918
894
402
363
232
232
100
91
55
54
33
29
24
18
17
16
12
12
11
9
8
7
5
4
4
4
2
2
1
1
1
1
1
1
1
1
1
1

ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Binding Specificity
2941

374

352

148

131

92

79

29

29

28

28

5

5

2

1

1

1

1

1

1

1

1

1

1

1
Whole Molecule
Reactivity
804

753

684

453

451

244

210

209

194

154

123

118

99

97

81

76

70

58

33

27

27

27

27

17

10

5

4

4

4

4

4

3

3

3

3

3

3

2

2

2

1

1

1

1
Rat
Host
1858

1738

628

417

153

99

30

26

25

20

19

12

11
Chicken

Specificity

Anti-Alkaline Phosphatase (calf intestine), anti-Chicken Serum, Rat IgG and Rat Serum

Purification

Immunoaffinity purified

Immunogen

Rat IgG whole molecule.

Type of Immunogen: Purified protein
Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 488) - Preadsorbed
WB, FLISA, FM, DB Polyclonal IgG Atto 488

Rabbit anti-Mouse IgG antibody (HRP) - Preadsorbed
ELISA, WB, IHC (fro), IHC (p), IHC (rs) Polyclonal IgG HRP F(ab')2 fragment

Goat anti-Rat IgG (Heavy Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP

Goat anti-Rat IgG (Heavy & Light Chain) antibody (HRP) - Preadsorbed
ELISA, IHC, WB Polyclonal IgG HRP

Goat anti-Rat IgG antibody (Cy3) - Preadsorbed
FACS, WB, FLISA, FM, DB Polyclonal IgG Cy3

Goat anti-Rat IgG antibody (DyLight 488)
WB, FLISA, FM Polyclonal IgG DyLight 488

Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 550) - Preadsorbed
WB, FLISA, FM Polyclonal IgG Atto 550

Goat anti-Rat IgG (Heavy & Light Chain) antibody (Atto 647N) - Preadsorbed
WB, FLISA, FM, DB Polyclonal IgG Atto 647N

Mouse anti-Rat IgG (Heavy & Light Chain) antibody (HRP)
ELISA, WB Polyclonal Mixture HRP

Goat anti-Rat IgG (Whole Molecule) antibody
ELISA, IHC, WB unconjugated F(ab')2 fragment

Application Notes

Approved: ELISA (1:1400 - 1:6100), IHC, WB

Usage: Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 μg of Rat IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) catalog no. NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1400 to 1:6100 of the stated concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment

Target Species of Antibody: Rat

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

0.05 M Tris chloride, 0.15 M sodium chloride, 0.001 M magnesium chloride, 0.0001 M zinc chloride, 50 % glycerol,  pH 8.0, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage

4 °C

Storage Comment

Store at 4°C. Do not freeze.
Target

IgG

Target Type

Antibody