Donkey anti-Sheep IgG (Whole Molecule) Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN301092

Quick Overview for Donkey anti-Sheep IgG (Whole Molecule) Antibody (Alkaline Phosphatase (AP)) (ABIN301092)

Target: IgG

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Product Details

Binding Specificity: Whole Molecule

Reactivity: Sheep

Host: Donkey

Specificity: Anti- Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Sheep IgG and Sheep Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins

Purification: Immunoaffinity purified

Immunogen: Sheep IgG whole molecule.

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA (1:3000 - 1:14000), IHC, WB

Usage: Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-anti body based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 μg of Sheep IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate catalog no. NPP-10 as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Sheep

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.05 M Tris chloride, 0.15 M sodium chloride, 0.001 M magnesium chloride, 0.0001 M zinc chloride, 50 % glycerol,  pH 8.0, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze.

Target Details

Target: IgG

Target Type: Antibody