Rabbit anti-Mouse IgG (Heavy Chain) Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN301145

Quick Overview for Rabbit anti-Mouse IgG (Heavy Chain) Antibody (Alkaline Phosphatase (AP)) (ABIN301145)

Target: IgG

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Product Details

Binding Specificity: Heavy Chain

Reactivity: Mouse

Host: Rabbit

Specificity: Anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum. Specificity was confirmed by ELISA at less than 1 % cross-reactivity against other mouse or human heavy or light chain isotypes

Purification: Immunoaffinity purified

Immunogen: Mouse IgG y (gamma heavy chain).

Application Details

Application Notes: Approved: ELISA (1:1000 - 1:11000), IHC (1:200 - 1:1000), WB (1:500 - 1:2500)

Usage: Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-anti body based enzymatic assays requiring lot-to-lot consistency. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.05 M Tris chloride, 0.15 M sodium chloride, 0.001 M magnesium chloride, 0.0001 M zinc chloride, 50 % glycerol,  pH 8.0, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze.

Target Details

Target: IgG

Target Type: Antibody