Rabbit anti-Cow IgG (Whole Molecule) Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN3045916

Quick Overview for Rabbit anti-Cow IgG (Whole Molecule) Antibody (Alkaline Phosphatase (AP)) (ABIN3045916)

Target: IgG

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: Immunoassay (IA)

Product Details

Binding Specificity: Whole Molecule

Reactivity: Cow

Host: Rabbit

Purpose: Rabbit anti-Bovine IgG (H&L) - Affinity Pure, ALP Conjugate

Specificity: Based on IEP, this antibody reacts with: • heavy (i³) chains on bovine IgG • light chains on all bovine immunoglobulins

Cross-Reactivity (Details): Based on IEP, no reactivity is observed to: • non-immunoglobulin bovine serum immunoglobulins

Purification: Affinity purified using solid phase Bovine IgG (H&L)

Purity: Affinity purified antibody is > 95 % based on SDS-PAGE. E 1 % at 280 nm = 13.0

Immunogen: Purified Bovine IgG, whole molecule

Application Details

Application Notes: This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator.
Suggested starting dilution(s): • 1:500-1:2,000 for Immunohistochemistry • 1:50,000-1:5,000 for ELISA/Western blot

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.5 mg/mL

Buffer: 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, contains 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: 1 year from date of receipt. Prepare working dilution prior to use and then discard.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody