Chicken anti-Rabbit IgG Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN336420

Quick Overview for Chicken anti-Rabbit IgG Antibody (Alkaline Phosphatase (AP)) (ABIN336420)

Target: IgG

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunoassay (IA)

Product Details

Reactivity: Rabbit

Host: Chicken

Purpose: Chicken anti-Rabbit IgG (H&L) - Affinity Pure, ALP Conjugate

Specificity: Based on IEP, this antibody reacts with: • heavy (i³) chains on rabbit IgG • light chains on all rabbit immunoglobulins

Cross-Reactivity (Details): Based on IEP, no reactivity is observed to: • non-immunoglobulin rabbit serum proteins

Purification: Affinity purified using solid phase Rabbit IgG

Purity: Affinity purified antibody is > 95 % based on SDS-PAGE. E 1 % at 280 nm = 13.2

Immunogen: Purified Rabbit IgG, whole molecule

Application Details

Application Notes: This conjugate is suitable for all immunoassay applications.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:500-1:2,000 for ELISA/Western blot 1:20. 1:2,000 for Immunohistochemistry 1:50. 1:5,000 for Immunocytochemistry

Comment:

Country of Origin: Ig fraction was prepared from Chicken eggs obtained from animals of US origin and under the care of a registered veterinarian.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.5 mg/mL

Buffer: 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, contains 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: 1 year from date of receipt. Prepare working dilution prior to use and then discard.

Storage: 4 °C,-20 °C

Storage Comment: Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody