Mouse anti-Human IgA Antibody

Catalog No. ABIN337297

Quick Overview for Mouse anti-Human IgA Antibody (ABIN337297)

Target: IgA

Clonality: Monoclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: Mc24-2E11

Isotype: IgG1

Product Details

Reactivity: Human

Host: Mouse

Specificity: Recognizes human immunoglobulin alpha heavy chains. No cross reactivity observed with IgG, IgM or free kappa and lambda light chains.

Purification: Protein G purified

Immunogen: Reduced and alkylated human IgA.

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Fr, IHC-P (10 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details

Target: IgA

Target Type: Antibody