Rabbit anti-Horse IgG (Fc Region) Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN338451

Quick Overview for Rabbit anti-Horse IgG (Fc Region) Antibody (Alkaline Phosphatase (AP)) (ABIN338451)

Target: IgG

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: Fc Region

Reactivity: Horse

Host: Rabbit

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Horse IgG, Horse IgG F(c) and Horse Serum. No reaction was observed against Horse IgG F (ab')2.

Purification: Immunoaffinity purified

Immunogen: Horse IgG F(c) fragment. Label: Alkaline Phosphatase (Calf Intestine) (MW)140kD).

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA (1:500 - 1:2000), IHC, WB

Usage: Suitable for use in immunoblot (western or dot blot), ELISA and immunohistochemistry. ELISA (standard capture): 1:500 to 1:2000, 1.0 μg of Horse IgG using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Horse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: TBS, 0.001 M Magnesium chloride, 0.0001 M Zinc chloride, 50 % glycerol,  pH 8.0 0.01 % sodium azide, 10 mg/mL BSA (IgG, protease free).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze.

Target Details

Target: IgG

Target Type: Antibody