Rabbit anti-Pig IgG (F(ab')2 Region) Antibody (HRP)

Catalog No. ABIN339182

Quick Overview for Rabbit anti-Pig IgG (F(ab')2 Region) Antibody (HRP) (ABIN339182)

Target: IgG

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: F(ab')2 Region

Reactivity: Pig

Host: Rabbit

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Swine IgG F (ab')2 and Swine Serum. No reaction was seen against Swine IgG F(c).

Purification: Immunoaffinity purified

Immunogen: Porcine IgG F (ab')2 fragment.

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA (1:5000 - 1:20000), IHC, WB

Usage: Suitable for use in Western Blot (Western or Dot Blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry. ELISA: 1:5000-1:20000, 1 μg of porcine IgG F (ab')2 was used in a standard capture ELISA with ABTS as a substrate for 30 minutes at RT. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Pig

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % Gentamicin sulfate.

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C,-20 °C

Storage Comment: Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.

Target Details

Target: IgG

Target Type: Antibody

Background: Synonyms: Porcine IgG Fab'2, Swine IgG Fab'2