Goat anti-Rabbit IgG Antibody (HRP)

Catalog No. ABIN363127

Quick Overview for Goat anti-Rabbit IgG Antibody (HRP) (ABIN363127)

Target: IgG

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Reactivity: Rabbit

Host: Goat

Specificity: Goat anti-Rabbit IgG conjugate is specific for rabbit IgG.

Purification: The antibody is obtained by affinity purified technique, which removes essentially all goat serum proteins, except the specific antibody for rabbit IgG. Goat anti-rabbit IgG is conjugated to peroxidase by means of a method described by Wilson MB and Nakane PK.

Immunogen: The antiserum was developed in goat using the rabbit IgG as the immunogen.

Application Details

Application Notes: ELISA: 1:5000approx. 1:20000, WB: 1:5000approx. 1:10000, IHC 1:1000approx. 5000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: HRP-labeled Goat IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl.

Handling Advice: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

References

Saito, Yamamoto, Mukaisho, Sato, Higo, Hattori, Yamamoto, Sugihara: "Mechanisms underlying cancer progression caused by ezrin overexpression in tongue squamous cell carcinoma." in: PLoS ONE, Vol. 8, Issue 1, pp. e54881, (2013) (PubMed).

Cheng, An, Yuan, Wang, Du, Yu, Zhang, Huang, Yang: "Hybrid expression cassettes consisting of a milk protein promoter and a cytomegalovirus enhancer significantly increase mammary-specific expression of human lactoferrin in transgenic mice." in: Molecular reproduction and development, Vol. 79, Issue 8, pp. 573-85, (2012) (PubMed).

Liu, Su, Sun, Wang, Qiao, Run, Liang: "Tau phosphorylation and μ-calpain activation mediate the dexamethasone-induced inhibition on the insulin-stimulated Akt phosphorylation." in: PLoS ONE, Vol. 7, Issue 4, pp. e35783, (2012) (PubMed).

Jia, Zhao, Zou, Suo: "Trypanosoma evansi: identification and characterization of a variant surface glycoprotein lacking cysteine residues in its C-terminal domain." in: Experimental parasitology, Vol. 127, Issue 1, pp. 100-6, (2011) (PubMed).

Xu, Zhao, Huang, Ding, Wang, Wang, Cheng, Kang, Wang: "CD40-expressing plasmid induces anti-CD40 antibody and enhances immune responses to DNA vaccination." in: The journal of gene medicine, Vol. 12, Issue 1, pp. 97-106, (2010) (PubMed).

Qian, Zhang, Cao, Liu: "Versatile immunosensor using a quantum dot coated silica nanosphere as a label for signal amplification." in: Analytical chemistry, Vol. 82, Issue 15, pp. 6422-9, (2010) (PubMed).

Target Details

Target: IgG

Target Type: Antibody