Mouse anti-Rat IgG2a Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN371222

Quick Overview for Mouse anti-Rat IgG2a Antibody (Alkaline Phosphatase (AP)) (ABIN371222)

Target: IgG2a

Clonality: Monoclonal

Conjugate: Alkaline Phosphatase (AP)

Application: Enzyme Immunoassay (EIA)

Clone: 2A 8F4

Isotype: IgG1

Product Details

Reactivity: Rat

Host: Mouse

Application Details

Application Notes: ELISA: 1/2,000-1/4,000.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 50 mM Tris/1 mM MgCl2/50 % Glycerol, pH 8.0 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details

Target: IgG2a

Target Type: Antibody

Background: There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). The IgG2a Ig subclass plays a critical role in the pathogenesis of humoral auto-immunity and protection against pathogens.Synonyms: Rat Immunoglobulin G2a