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Mouse anti-Rat IgG2a Antibody (Cy5)

FACS, IF Monoclonal 2A 8F4 IgG1 Cy5
Catalog No. ABIN371224
  • Target See all IgG2a products
    IgG2a
    Reactivity
    • 98
    • 18
    • 1
    Rat
    Host
    • 57
    • 29
    • 13
    • 13
    • 5
    Mouse
    Clonality
    • 72
    • 26
    Monoclonal
    Conjugate
    • 22
    • 13
    • 13
    • 10
    • 9
    • 5
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cy5
    Application
    • 58
    • 46
    • 38
    • 33
    • 24
    • 19
    • 11
    • 10
    • 7
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF)
    Clone
    2A 8F4
    Isotype
    IgG1
  • Application Notes
    Flow Cytometry: < / = 0.1 μg/10^6 cells. Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.

    Other applications not tested.
    Otimal dilutions are dependent on conditions and should be determined by the user.
    Comment

    Excitation / Emission wavelength: 652 nm / 672 nm

    Restrictions
    For Research Use only
  • Concentration
    0.1 mg/mL
    Buffer
    PBS containing 0.09 % Sodium Azide as preservative.
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freezing and thawing.
    Protect conjugated products from light.
    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or in (aliquots) at -28 °C for longer.
  • Target
    IgG2a
    Abstract
    IgG2a Products
    Target Type
    Antibody
    Background
    There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). The IgG2a Ig subclass plays a critical role in the pathogenesis of humoral auto-immunity and protection against pathogens.Synonyms: Rat Immunoglobulin G2a
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