Mouse anti-Rat IgM (Chain mu) Antibody
Func
Monoclonal
M 2A1
IgG1
unconjugated
F(ab')2 fragment
1 image
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- Target See all IgM products
- IgM
- Fragment
- F(ab')2 fragment
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Binding Specificity
- Chain mu
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Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Application
- Functional Studies (Func)
- Purification
- Purified
- Clone
- M 2A1
- Isotype
- IgG1
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- Application Notes
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B Cell Stimulation: 5 μg per mL of tissue culture media.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.
- Preservative
- Without preservative
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
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- Target
- IgM
- Abstract
- IgM Products
- Target Type
- Antibody
- Background
- IgM normally constitutes about 10 % of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences. Class specific anti immunoglobulin antibodies are useful for: The characterization of malignant B cell proliferations. All but acute lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenström's disease bear intracytoplasmic IgM. The other isotypes are less frequently found. On the other hand multiple myelomas are usually of the IgG or IgA type. Characterization of plasma cells in inflammatory conditions: Plasma cell typing can be of use for the classification of intestinal inflammatory conditions such as inflammatory bowel disease and allergic conditions. In the latter a specific increase in the number of IgE plasma cells can be demonstrated.Synonyms: Rat Immunoglobulin M
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