Goat anti-Mouse IgG Antibody (Alkaline Phosphatase (AP)) - Preadsorbed

Catalog No. ABIN375655

Quick Overview for Goat anti-Mouse IgG Antibody (Alkaline Phosphatase (AP)) - Preadsorbed (ABIN375655)

Target: IgG

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISpot

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Goat

Purpose: Goat Anti-Mouse IgG, Human ads-AP

Specificity: Reacts with mouse IgG 1, IgG 2a, IgG 2b and IgG 3 as demonstrated by ELISA and flow cytometry.

Characteristics: Goat Anti-Mouse IgG-AP antibody with minimal reactivity to human proteins for use in ELISA, immunohistochemistry / immunocytochemistry, western blotting, and ELISpot assays.

Purification:

Purified by affinity chromatography on mouse IgG covalently linked to agarose, cross-adsorbed against mouse IgM and IgA / human immunoglobulins and pooled sera for minimal reactivity

Preadsorption: Human pre-adsorbed

Immunogen: Mouse IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pooled antisera from goats hyperimmunized with mouse IgG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM Tris/1 mM MgCl2/50 % Glycerol containing < 0.1 % sodium azide, pH 8.0

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C

References

Quinn, Quirion, Lo, Misplon, Epstein, Chiorini: "Intranasal Administration of Adeno-associated Virus Type 12 (AAV12) Leads to Transduction of the Nasal Epithelia and Can Initiate Transgene-specific Immune Response." in: Molecular therapy : the journal of the American Society of Gene Therapy, (2011) (PubMed).

Target Details

Target: IgG

Target Type: Antibody

Background: Optional[Description]: Goat Anti-Mouse IgG, Human ads-AP