Goat anti-Mouse IgG1 Antibody (Alkaline Phosphatase (AP)) - Preadsorbed

Catalog No. ABIN375662

Quick Overview for Goat anti-Mouse IgG1 Antibody (Alkaline Phosphatase (AP)) - Preadsorbed (ABIN375662)

Target: IgG1

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISpot, Immunohistochemistry (Whole Mount) (IHC (wm))

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Goat

Purpose: Goat Anti-Mouse IgG1, Human ads-AP

Specificity: Reacts with the heavy chain of mouse IgG 1 as demonstrated by ELISA and flow cytometry.

Characteristics: Goat Anti-Mouse IgG1-AP antibody with minimal reactivity to human proteins for use in ELISA, immunohistochemistry / immunocytochemistry, western blotting, and ELISpot assays.

Purification:

Purified by affinity chromatography on mouse IgG1 covalently linked to agarose, cross-adsorbed against mouse IgG2a, IgG2b, IgG3, IgM, and IgA / human immunoglobulins and pooled sera for minimal reactivity

Preadsorption: Human pre-adsorbed

Immunogen: Mouse IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pooled antisera from goats hyperimmunized with mouse IgG1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM Tris/1 mM MgCl2/50 % Glycerol containing < 0.1 % sodium azide, pH 8.0

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C

References

Conter, Song, Shlomchik, Tomayko: "CD73 expression is dynamically regulated in the germinal center and bone marrow plasma cells are diminished in its absence." in: PLoS ONE, Vol. 9, Issue 3, pp. e92009, (2014) (PubMed).

Target Details

Target: IgG1

Target Type: Antibody

Background:

Description: Goat Anti-Mouse IgG1, Human ads-AP

NCBI_Official_Symbol: Ighg1

Gene ID: 16017

UniProt: P01868