Goat anti-Mouse IgM Antibody (Biotin) - Preadsorbed

Catalog No. ABIN375906

Quick Overview for Goat anti-Mouse IgM Antibody (Biotin) - Preadsorbed (ABIN375906)

Target: IgM

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Flow Cytometry (FACS), Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISpot, Immunoelectron Microscopy (IEM)

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Goat

Purpose: Goat Anti-Mouse IgM, Human ads-BIOT

Specificity: Reacts with the heavy chain of mouse IgM as demonstrated by ELISA and flow cytometry.

Characteristics: Goat Anti-Mouse IgM-Biotin antibody with minimal reactivity to human proteins for use in ELISA, flow cytometry, immunohistochemistry / immunocytochemistry, electron microscopy, western blotting, immunoprecipitation, and ELISpot assays.

Purification:

Purified by affinity chromatography on mouse IgM covalently linked to agarose, cross-adsorbed against mouse IgG and IgA / human immunoglobulins and pooled sera for minimal reactivity

Preadsorption: Human pre-adsorbed

Immunogen: Mouse IgM

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pooled antisera from goats hyperimmunized with mouse IgM

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline containing < 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C

References

Andersson, Dahlgren: "2-Hydroxyethyl methacrylate (HEMA) promotes IgG but not IgM antibody production in vivo in mice." in: European journal of oral sciences, Vol. 119, Issue 4, pp. 305-9, (2011) (PubMed).

Palmer, Stasko, Waters, Thacker: "Examination of the reticular epithelium of the bovine pharyngeal tonsil." in: Anatomical record (Hoboken, N.J. : 2007), Vol. 294, Issue 11, pp. 1939-50, (2011) (PubMed).

Target Details

Target: IgM

Target Type: Antibody

Background:

Description: Goat Anti-Mouse IgM, Human ads-BIOT

NCBI_Official_Symbol: Ighm

Gene ID: 16019

UniProt: P01872