Goat anti-Mouse Ig Antibody (HRP) - Preadsorbed

Catalog No. ABIN376237

Quick Overview for Goat anti-Mouse Ig Antibody (HRP) - Preadsorbed (ABIN376237)

Target: Ig

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), ELISpot

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Goat

Purpose: Goat Anti-Mouse Ig, Human ads-HRP

Specificity: Reacts with the heavy and light chains of mouse IgM, IgG 1, IgG 2a, IgG 2b, IgG 3 and IgA as demonstrated by ELISA and flow cytometry.

Characteristics: Goat Anti-Mouse Ig-HRP antibody with minimal reactivity to human proteins for use in ELISA, immunohistochemistry / immunocytochemistry, western blotting, and ELISpot assays.

Purification:

Purified by affinity chromatography on mouse IgG, IgM, and IgA covalently linked to agarose, cross-adsorbed against human immunoglobulins and pooled sera for minimal reactivity

Preadsorption: Human pre-adsorbed

Immunogen: Mouse IgG, IgM, and IgA

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pooled antisera from goats hyperimmunized with mouse IgG, IgM, and IgA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 50 % Glycerol/50 % Phosphate buffered saline, pH 7.4

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C

Storage Comment: 2-8°C

References

Zantow, Just, Lagkouvardos, Kisling, Dübel, Lepage, Clavel, Hust: "Mining gut microbiome oligopeptides by functional metaproteome display." in: Scientific reports, Vol. 6, pp. 34337, (2016) (PubMed).

Target Details

Target: Ig