Goat anti-Mouse IgG1 Antibody (PE) - Preadsorbed

Catalog No. ABIN376449

Quick Overview for Goat anti-Mouse IgG1 Antibody (PE) - Preadsorbed (ABIN376449)

Target: IgG1

Clonality: Polyclonal

Conjugate: PE

Application: Flow Cytometry (FACS), FLISA, Multiplex Assay (MA)

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Goat

Purpose: Goat Anti-Mouse IgG1, Human ads-PE

Specificity: Reacts with the heavy chain of mouse IgG1

Characteristics: Goat Anti-Mouse IgG1-PE antibody with minimal reactivity to human proteins for use in flow cytometry and multiplex assays.

Purification:

Purified by affinity chromatography on mouse IgG1 covalently linked to agarose, cross-adsorbed against mouse IgG2a, IgG2b, IgG3, IgM, and IgA / human immunoglobulins and pooled sera for minimal reactivity

Preadsorption: Human pre-adsorbed

Immunogen: Mouse IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pooled antisera from goats hyperimmunized with mouse IgG1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline containing < 0.1 % sodium azide and a stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C

References

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Ratiu, Racine, Hasham, Wang, Branca, Chapman, Zhu, Donghia, Philip, Schott, Wasserfall, Atkinson, Mills, Leeth, Serreze: "Genetic and Small Molecule Disruption of the AID/RAD51 Axis Similarly Protects Nonobese Diabetic Mice from Type 1 Diabetes through Expansion of Regulatory B Lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 198, Issue 11, pp. 4255-4267, (2017) (PubMed).

Priglinger, Szober, Priglinger, Merl, Euler, Kernt, Gondi, Behler, Geerlof, Kampik, Ueffing, Hauck: "Galectin-3 Induces Clustering of CD147 and Integrin-β1 Transmembrane Glycoprotein Receptors on the RPE Cell Surface." in: PLoS ONE, Vol. 8, Issue 7, pp. e70011, (2013) (PubMed).

Hasham, Snow, Donghia, Branca, Lessard, Stavnezer, Shopland, Mills: "Activation-induced cytidine deaminase-initiated off-target DNA breaks are detected and resolved during S phase." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 5, pp. 2374-82, (2012) (PubMed).

Volná, Jarjour, Baxter, Roffler, Monnat, Stoddard, Scharenberg: "Flow cytometric analysis of DNA binding and cleavage by cell surface-displayed homing endonucleases." in: Nucleic acids research, Vol. 35, Issue 8, pp. 2748-58, (2007) (PubMed).

Target Details

Target: IgG1

Target Type: Antibody

Background:

Description: Goat Anti-Mouse IgG1, Human ads-PE

NCBI_Official_Symbol: Ighg1

Gene ID: 16017

UniProt: P01868