Goat anti-Chicken IgG (Fab Region) Antibody

Catalog No. ABIN457622

Quick Overview for Goat anti-Chicken IgG (Fab Region) Antibody (ABIN457622)

Target: IgG

Clonality: Polyclonal

Application: Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)

Isotype: IgG

Product Details

Binding Specificity: Fab Region

Reactivity: Chicken

Host: Goat

Purpose: Goat anti Chicken IgG Fab

Specificity: Precipitating polyclonal Goat antiSerum to Chicken Fab of IgG

Cross-Reactivity (Details): Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since homologous proteins of different species frequently share antigenic determinants. Cross- reactivity of this antiSerum has not been tested in detail.

Characteristics: The antiserum is reacting with the Fab subunits IgG, IgA, IgM and other Ig classes of both light chain type, with their Fab or F(ab')2 subunits and with free light chains of kappa and lambda type. It does not react with any non-Ig protein in chicken serum, as tested by immunoelectro- phoresis and double radial immunodiffusion. In precipitating techniques as immunoelectrophoresis and single and double radial immuno- diffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins, as secondary antibody to precipitate the immunoglobulin in normal chicken serum.

Purification: Delipidated, heat inactivated lyophilized stable whole serum

Immunogen: Purified Fab of normal IgG isolated from pooled chicken serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: Precipitation assays. In immunoelectrophoresis use 2 μL or equivalent against 120 μL antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and diluted) in 2 mm diameter peripheral wells

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated, lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled goat seru

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details

Target: IgG

Target Type: Antibody